Production of a 135-Residue Long N-Truncated Human Keratinocyte Growth Factor 1 in Escherichia Coli

    May 2022 in “ Research Square (Research Square) ”
    Young Su Kim, Hye‐Jeong Lee, Gabriella Aphrodita Handoko, Jae-hui Kim, Seong-Bo Kim, Min-ho Won, Jung-ho Park, Jungoh Ahn
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    TLDR KGF-1 135 is a stable and effective alternative for treating oral mucositis.
    In this study, researchers produced and purified a 135-residue N-truncated variant of human keratinocyte growth factor 1 (KGF-1) in Escherichia coli, aiming to improve production yield and evaluate its biological activity. The variant was expressed as a fusion with thioredoxin and purified to high purity (>99%). Circular dichroism spectroscopy confirmed that the structure of KGF-1 135 was similar to the original KGF-1, and cell proliferation assays demonstrated its biological activity. The study concluded that KGF-1 135 could be a stable and effective alternative to the existing KGF-1 used for preventing and treating oral mucositis.
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