research Analytical Method for the Biomonitoring of Bisphenols and Parabens by Liquid Chromatography Coupled to Tandem Mass Spectrometry in Human Hair
This study developed and validated a high-performance liquid chromatography coupled to tandem mass spectrometry method for detecting bisphenol A, its chlorinated derivatives, bisphenol F, bisphenol S, and parabens in human hair. The method, which includes acidic hydrolysis and liquid-liquid extraction, showed high linearity (over 0.996), trueness, and precision with intra-day and inter-day bias values ranging from 86% to 118% and relative standard deviation values from 0% to 19%. Limits of quantification were set at 0.25 ng/g for bisphenol A and parabens, 0.05 ng/g for bisphenols F and S, and 0.00625 ng/g for chlorinated derivatives of bisphenol A. This method was successfully applied to hair samples from hospital professionals, marking the first quantification of chlorinated derivatives of bisphenol A in hair.
