Analytical Method for the Biomonitoring of Bisphenols and Parabens by Liquid Chromatography Coupled to Tandem Mass Spectrometry in Human Hair

The study developed and validated a highly sensitive UHPLC-MS/MS method for detecting bisphenols (BPA, BPF, BPS, and chlorinated derivatives) and parabens in human hair, providing a means to assess long-term exposure to these endocrine disruptors. The method was optimized for accuracy and reliability and applied to hair samples from five hospital professionals, revealing widespread exposure. BPA and BPS were found in all samples, with mean concentrations of 2.237 ± 3.077 ng/g and 2.932 ± 2.631 ng/g, respectively, while methylparaben was the most prevalent paraben at 69.735 ± 139.538 ng/g. This method could be used to monitor exposure in vulnerable populations and link it to health conditions.