Functional Analysis of Vitamin D Receptor Using Adenovirus Vector

February 2023 in “ Journal of steroid biochemistry and molecular biology/The Journal of steroid biochemistry and molecular biology ”

Satoko Kise, Ayano Iijima, Chika Nagao, Tadashi Okada, Hiroki Mano, Miyu Nishikawa, Shinichi Ikushiro, Yoshitsugu Kanemoto, Shigeaki Kato, Teruyuki Nakanishi, Shigeto Sato, Kaori Yasuda, Toshiyuki Sakaki

Vitamin D receptor VDR alopecia adenovirus vectors AdVs VDR mutations split luciferase system ligand-binding ability nuclear translocation HaCaT-VDR-KO cells CYP24A1 transcription qPCR VDR-RXR interaction domain ligand-binding domain hair loss

TLDR The study found that certain mutations in the vitamin D receptor can cause rickets and potentially affect hair growth.

The study investigated the function of the Vitamin D receptor (VDR) and its relationship with alopecia using adenovirus vectors (AdVs). The researchers generated genetically modified rats with different VDR mutations and used three systems to evaluate the VDRs: a split luciferase system to examine ligand-binding ability, investigation of ligand-dependent nuclear translocation in HaCaT-VDR-KO cells, and examination of CYP24A1 transcription induction by ligand addition using qPCR. The study found that mutations in the VDR-RXR interaction domain can result in alopecia, while mutations in the ligand-binding domain do not. The study concluded that AdVs expressing VDR could be a promising tool for elucidating VDR functions, particularly in relation to alopecia.

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Research cited in this study

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research Functional Analyses of a Novel Missense and Other Mutations of the Vitamin D Receptor in Association with Alopecia

20 citations , July 2017 in “Scientific Reports”

A single amino acid change in the vitamin D receptor can disrupt its function and lead to hair loss.

research The Role of Vitamin D Receptor Mutations in the Development of Alopecia

109 citations , June 2011 in “Molecular and Cellular Endocrinology”

Vitamin D receptor mutations can cause alopecia by affecting hair growth genes.

research Disruption of the Hedgehog Signaling Pathway Contributes to Hair Follicle Cycling Deficiency in VDR Knockout Mice

53 citations , May 2010 in “Journal of Cellular Physiology”

Mice without Vitamin D receptors have hair growth problems because of issues in the hedgehog signaling pathway.

research Vitamin D and Human Health: Lessons from Vitamin D Receptor Null Mice

1533 citations , October 2008 in “Endocrine reviews”

Mice without the vitamin D receptor have bone issues and other health problems, suggesting vitamin D is important for preventing various diseases in humans.

research Targeting Expression of the Human Vitamin D Receptor to the Keratinocytes of Vitamin D Receptor Null Mice Prevents Alopecia

115 citations , December 2001 in “Endocrinology”

Expressing the human vitamin D receptor in skin cells prevents hair loss in certain mice.

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community My Theory Regarding a Cause and Cure for Androgenetic Alopecia

in Treatment 102 upvotes 5 years ago

Hair loss theory suggests imbalance between Vitamin D Receptor (VDR) and Androgen Receptor (AR) activation. Proposed treatment includes upregulating VDR, downregulating AR, and improving mitochondrial health.

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The conversation discusses hair loss treatments, including oral finasteride, topical minoxidil, and microneedling, and the potential impact of Vitamin D and folic acid deficiencies on hair regrowth. It suggests that while male pattern baldness is the primary cause, addressing vitamin deficiencies may aid in improving regrowth.

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in Research/Science 9 upvotes 1 year ago

Topical Vitamin D3 may stimulate hair growth and has been used for Alopecia Areata. There is a question about the lack of research on its use for Androgenetic Alopecia (AGA).

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community Compressed part of research of theory of androgenic/anabolitic balance. AGA h-responders analytic. Theory of physio-metabolitic method of anti AGA treatment

in Research 865 upvotes 1 year ago

The treatment for androgenetic alopecia involves using finasteride and minoxidil with intense exercise and cold exposure to boost metabolism and reduce androgenic effects, potentially leading to hair regrowth. This approach may activate biological pathways for improved hair and overall health.

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community Supplements for Health hair as for Gemini & Grok

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The conversation discusses various supplements and vitamins like Vitamin D3, K2, Biotin, Iron, Zinc, and B Vitamins for improving hair health. It also mentions a combination of Tocotrienols, Pycnogenol, Saw Palmetto, Pumpkin Seed Oil, Stinging Nettle, and Myricetin for stabilizing hair, with Saw Palmetto, Pygeum, and Stinging Nettle noted for thickening hair despite side effects.

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research Gene Therapy for Alopecia in Type II Rickets Model Rats Using Vitamin D Receptor-Expressing Adenovirus Vector

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Gene therapy helped rats with a specific type of rickets grow hair without severe inflammation.

research The Role of Vitamin D Receptor Signaling in Hair Follicle Health and Alopecia: Current Understanding and Therapeutic Implications

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Vitamin D receptor is crucial for hair health and may help treat hair loss.

Functional Analysis of Vitamin D Receptor Using Adenovirus Vector

Research cited in this study

research Functional Analyses of a Novel Missense and Other Mutations of the Vitamin D Receptor in Association with Alopecia

research The Role of Vitamin D Receptor Mutations in the Development of Alopecia

research Disruption of the Hedgehog Signaling Pathway Contributes to Hair Follicle Cycling Deficiency in VDR Knockout Mice

research Vitamin D and Human Health: Lessons from Vitamin D Receptor Null Mice

research Targeting Expression of the Human Vitamin D Receptor to the Keratinocytes of Vitamin D Receptor Null Mice Prevents Alopecia

Related Community Posts Join

community My Theory Regarding a Cause and Cure for Androgenetic Alopecia

community Is Vitamin D the cause of my baldness or slow regrowth?

community Why are there no studies on Topical Vitamin D3 for AGA?

community Compressed part of research of theory of androgenic/anabolitic balance. AGA h-responders analytic. Theory of physio-metabolitic method of anti AGA treatment

community How a follicle next to it is so genetically different that it never miniaturized??

community Supplements for Health hair as for Gemini & Grok

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research Gene Therapy for Alopecia in Type II Rickets Model Rats Using Vitamin D Receptor-Expressing Adenovirus Vector

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