Functional Analysis of Vitamin D Receptor Using Adenovirus Vector
TLDR The study found that certain mutations in the vitamin D receptor can cause rickets and potentially affect hair growth.
The study investigated the function of the Vitamin D receptor (VDR) and its relationship with alopecia using adenovirus vectors (AdVs). The researchers generated genetically modified rats with different VDR mutations and used three systems to evaluate the VDRs: a split luciferase system to examine ligand-binding ability, investigation of ligand-dependent nuclear translocation in HaCaT-VDR-KO cells, and examination of CYP24A1 transcription induction by ligand addition using qPCR. The study found that mutations in the VDR-RXR interaction domain can result in alopecia, while mutations in the ligand-binding domain do not. The study concluded that AdVs expressing VDR could be a promising tool for elucidating VDR functions, particularly in relation to alopecia.
