Upregulation of Genes Orchestrating Keratinocyte Differentiation, Including the Novel Marker Gene ID2, by Contact Sensitizers in Human Bulge-Derived Keratinocytes

January 2010 in “ Journal of Biochemical and Molecular Toxicology ”

Yoshie Yoshikawa, Yusuke Sasahara, Yukio Kitano, Nozomi Kanazawa, Hiroki Shima, Tomoko Hashimoto‐Tamaoki

keratinocyte differentiation ID2 NRF2-mediated signaling pathway IL1B IL8 human bulge-derived keratinocytes contact sensitizers irritants

TLDR The ID2 gene can help distinguish between sensitizers and irritants in skin cells.

The study investigated the molecular responses of human bulge-derived keratinocytes (BDKs) to contact sensitizers and irritants, comparing them with responses in the THP-1 cell line. It was found that BDKs showed high reactivity to both sensitizers and irritants, with the NRF2-mediated signaling pathway being upregulated in response to sensitizers. Notably, the expression of IL1B and IL8 genes was induced by sensitizers in THP-1, but in BDKs, these genes were more strongly induced by irritants. Additionally, genes involved in keratinocyte differentiation, including the novel marker gene ID2, were significantly upregulated in BDKs in response to sensitizers, suggesting that ID2 could serve as a novel marker for distinguishing between sensitizers and irritants in vitro.

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