LB1027 Identifying the Mechanism of Tension-Associated Tissue Fibrosis Based on Single Cell RNA Sequencing on Keloid Scar

This study investigates the role of mechanical stretching in keloid scar formation using single-cell RNA sequencing. Keloids were categorized into high-tension (chest, sternum, back) and low-tension (earlobes) groups. High-tension areas showed a significant presence of pro-inflammatory fibroblasts and upregulated genes related to stress responses and inflammation, particularly the AP-1 signaling pathway. In vitro models confirmed that mechanical stretching activates the CXCL12 and AP-1 pathways, increasing the expression of fibrosis-related genes like MMP-2 and MMP-9. These findings were consistent in patient-derived keloid fibroblasts from high-tension areas, which also presented more aggressive symptoms and higher recurrence risk. The study highlights the link between mechanical tension and keloid pathology, suggesting potential targets for therapy.