Spheroid Cell Cultures as a Promising Study Model for Hair Follicle Regeneration

    April 2018 in “ Journal of Investigative Dermatology ”
    F. Oppenheimer, Julieta María Ceruti, Gustavo José Leirós, María Eugenia Balañá
    Image of study
    TLDR Culturing Dermal Papilla Cells and Hair Follicle Stem Cells in 3D conditions can significantly improve hair regeneration potential.
    Five years ago, a study was conducted to test different culture conditions for Dermal Papilla Cells (DPC) and Hair Follicle Stem Cells (HFSC) with the aim of regenerating hair follicles in skin substitutes. The study found that 3D-culture strategies were necessary as DPC's inductive ability was lost when cultured in a monolayer. The results showed that Alkaline phosphatase activity (APa), which correlates with hair inductive potential of DPC, was 25 times higher in spheroids than in monolayer cultured cells. Additionally, VEGF and Angiogenin, which promote skin angiogenesis and hair cycle, showed an 8-fold higher expression in DPC spheroids. After 48 hours of culture time, an 8-fold increase of Wnt agonists and a 4-fold decrease of Dkk1 expression was observed. The study concluded that the culture conditions were crucial in obtaining DPC-spheroids with improved inductivity and heterotypic spheroids with histological features reminiscent of embryonic hair-buds.
    Discuss this study in the Community →

    Related Community Posts Join

    6 / 1000+ results
      Diffuse hair loss and scalp biopsies

      community Diffuse hair loss and scalp biopsies

      in Research  692 upvotes 4 months ago
      Scalp biopsies are crucial for diagnosing hair loss conditions like Diffuse Unpatterned Alopecia (DUPA) and retrograde hair loss, as treatments like finasteride and dutasteride may not be effective if other conditions are present. Combining PPAR-GAMMA agonists with retinoids could improve treatments for conditions like Lichen Planopilaris.

      community Keratin microspheres - breakthrough

      in Research/Science  13 upvotes 9 months ago
      A gel of keratin microspheres promotes hair follicle growth, showing similar effectiveness to minoxidil in mice. The treatment activates hair growth pathways and reduces inflammation, with potential applications in drug delivery for hair-related disorders.

      community Fats on your Head to Stop Hair Loss?

      in Research/Science  142 upvotes 10 months ago
      The conversation discusses the potential of long-chain unsaturated fatty acids, like oleic and linoleic acid, as an additional treatment for hair loss, which may inhibit the enzyme responsible for converting testosterone to DHT and promote hair growth. Users humorously suggest using oils topically and discuss other hair loss treatments, but the main focus is on the science behind fatty acids and their role in hair health.

      community New and Interesting HairLoss Studies/Papers/Reviews

      in Treatment  42 upvotes 4 years ago
      Hair loss treatments discussed include Dutasteride with Ketoconazole, tissue engineering strategies, and androgenetic alopecia therapies. Massage doubles follicular retention, improving treatment effectiveness.

    Similar Research

    6 / 1000+ results