Screen Steroid 5 Alpha-Reductase Inhibitors In Vitro By High Performance Liquid Chromatography

    January 2005 in “ Fuzhou daxue xuebao. Ziran kexue ban ”
    Jun Xie
    TLDR The method is quick and effective for testing steroid 5 alpha-reductase inhibitors.
    The study developed an in vitro method using high performance liquid chromatography to screen steroid 5 alpha-reductase inhibitors. Testosterone and either Finasteride or Epristeride were incubated, and the samples were analyzed to determine the enzymatic activity and inhibition constant (K_i) using Lineweaver-Burk plots. The K_i values were found to be 4.32±3.8 μmol/L for Finasteride and 1.14±0.23 μmol/L for Epristeride. The method was concluded to be rapid and efficient for screening these inhibitors.
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