Screen Steroid 5 Alpha-Reductase Inhibitors In Vitro By High Performance Liquid Chromatography
January 2005
in “
Fuzhou daxue xuebao. Ziran kexue ban
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New to Finasteride? There is a guide in the encyclopedia. Read the guide → Studysummary This study developed a rapid and efficient in vitro method using high-performance liquid chromatography to screen for steroid 5 alpha-reductase inhibitors, determining inhibition constants for Finasteride and Epristeride.
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The study developed an in vitro method using high performance liquid chromatography to screen steroid 5 alpha-reductase inhibitors. Testosterone and either Finasteride or Epristeride were incubated, and the samples were analyzed to determine the enzymatic activity and inhibition constant (K_i) using Lineweaver-Burk plots. The K_i values were found to be 4.32±3.8 μmol/L for Finasteride and 1.14±0.23 μmol/L for Epristeride. The method was concluded to be rapid and efficient for screening these inhibitors.
