Simultaneous RP-HPLC-DAD Determination of Dansyl Amino Acids in Chemically Treated Human Hair

    December 2018 in “ Macedonian Journal of Chemistry and Chemical Engineering ”
    Pece Sherovski, Марина Стефова, Natasha Ristovska
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    TLDR A new method accurately measures amino acids in treated hair, showing bleaching reduces amino acids while protein treatments increase them.
    A reliable RP-HPLC-DAD method was developed for analyzing 19 amino acids in chemically treated hair, showing improved peak resolution and excellent linearity in the 0.05-0.5 mmol/l range. The study found that amino acid concentrations, especially cystine, decreased in bleached hair with higher hydrogen peroxide levels, while protein treatments increased typical keratin amino acids. This method proved useful for amino acid analysis in protein hydrolysates and could be applied to other protein samples.
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