Residual Antibacterial Activity of Canine Hair Treated with Topical Antimicrobial Sprays Against Staphylococcus Pseudintermedius In Vitro

The study investigated the residual antibacterial activity of canine hairs treated with four different topical antimicrobial sprays against Staphylococcus pseudintermedius. Twelve dogs with no dermatological disease were treated with sprays containing varying concentrations and combinations of chlorhexidine, miconazole, and TrizEDTA. Hairs were collected and tested for bacterial growth inhibition over 10 days. Results showed significant differences in inhibition zones, with spray B (2% miconazole nitrate, 2% chlorhexidine gluconate, TrizEDTA) producing the largest zones, followed by sprays D, C, and A. All sprays maintained antimicrobial activity for 10 days, indicating that the efficacy of these sprays depends on the concentration and combination of active ingredients, which could be beneficial for treating canine pyoderma.