Rat Hair Follicle Stem Cell-Derived Exosomes: Isolation, Characterization, and Comparative Analysis of Their In Vitro Wound Healing Potential

This study successfully isolated and characterized exosomes from rat hair follicle stem cells (rHFSCs) and evaluated their wound-healing potential. The exosomes, confirmed to have a spherical morphology and specific elemental composition, showed significant pro-regenerative potential in vitro. Protein quantification revealed higher protein content in the secretome compared to exosomes, but both demonstrated therapeutic relevance. Functional analysis of 30 wound-healing biomolecules and scratch assays indicated significant wound closure, highlighting the potential of rHFSC-derived exosomes and secretome as innovative, cell-free therapeutic agents for cutaneous regeneration and their broader applicability in regenerative medicine.