In Vivo and In Silico Evaluation of Petroselinum Crispum Leaf Fractions as Anti-Alopecia

The study investigates the anti-alopecia potential of Petroselinum crispum (parsley) leaf fractions using in vivo and in silico methods. Androgenetic alopecia (AGA) is linked to the activation of androgen receptors, which shortens the hair growth cycle's anagen phase. The study used a rabbit model and molecular docking to evaluate the efficacy of parsley leaf ethyl acetate fractions. Fractions 2 and 4 showed significant hair growth promotion, with hair length and weight increases comparable to minoxidil. LC-MS analysis identified several compounds, with paeonilactone C1 showing the highest binding energy to androgen receptors. These findings suggest that parsley leaf fractions could be developed into a hair tonic for alopecia treatment and prevention.