Research Techniques Made Simple: Optical Clearing and Three-Dimensional Volumetric Imaging of Skin Biopsies
June 2020
in “
Journal of Investigative Dermatology
”
three-dimensional volumetric imaging skin histology hair follicles sebaceous glands optical clearing solvent-based approaches aqueous-based approaches fixation permeabilization immunostaining light-sheet fluorescence microscope two-photon microscope melanin 3D imaging skin biopsy oil glands solvent-based methods water-based methods light-sheet microscope
Studysummary This article reviews the steps and methods involved in preparing skin tissues for three-dimensional volumetric imaging, highlighting its potential to provide detailed insights into skin structure not possible with traditional 2D histology, but reports no new findings.
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