New Insight Into the Immunostaining Method Suitable for the Pilosebaceous Complex Unit

January 2016 in “ The American journal of dermatopathology/American journal of dermatopathology ”

Jae-In Lee, Jongman Yoo, Hyung Min Chung

immunostaining pilosebaceous complex peroxidase-labeled conjugated secondary antibody LSAB method immunohistochemistry hair follicle sebaceous glands bulge regions IHC SGs HFs

TLDR The study found that a one-step antibody method is better than the LSAB method for accurately studying hair follicle structures without false positives.

This study demonstrated that the 1-step, peroxidase-labeled conjugated secondary antibody method was more effective than the 2-step LSAB method for immunohistochemistry (IHC) of pilosebaceous units. The LSAB method, despite its signal amplification advantage, led to poor differential immunoreactivity (IR) separation and false-positive staining in sebaceous glands (SGs). In contrast, the 1-step method provided more distinct IR patterns without false positives, making it preferable for accurately characterizing and localizing hair follicle (HF) substructures, particularly the bulge regions. This finding suggested that the 1-step method should be the IHC method of choice for studying pilosebaceous units.

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