Mitochondrial Oxygen Consumption and Immunocytochemistry of Human Dental Pulp Stem Cell Following 808 nm PBM Therapy: A 3D Cell Culture Study

This study explores the impact of 808 nm photobiomodulation (PBM) therapy on human dental pulp stem cells (hDPSCs) in a 3D cell culture setting, focusing on mitochondrial oxygen consumption and immunocytochemistry. The research shows that PBM can enhance mitochondrial function and promote osteogenic differentiation, which are vital for tissue regeneration. Using a 96-well microplate design, the study tested PBM doses of 5 J/cm² and 15 J/cm², finding that PBM modulates mitochondrial activity and osteogenic markers in a dose-dependent manner. Results indicate increased cell viability and mitochondrial respiration, with variable protein expression changes, suggesting PBM's potential in improving regenerative medicine outcomes. The study calls for further research to optimize PBM dosing strategies for clinical applications.