Use of Mass Spectrometry Imaging and a Full Thickness 3D Skin Equivalent for Evaluation of Percutaneous Absorption

The document reports on studies investigating topical treatments for skin conditions, including atopic dermatitis (AD), psoriasis (PSO), and androgenetic alopecia (AGA). SB414, a nitric oxide-releasing formulation, was effective in reducing ear swelling and bacterial counts in animal models, suggesting its use for AD. SIG-1451, an IPC analog, showed promise in reducing pro-inflammatory cytokines and in vivo inflammation, indicating potential for AD treatment. Mass spectrometry imaging (MSI) revealed differences in the percutaneous absorption of retinoic acid, comparing a solution and a commercial formulation. SM04554, a Wnt signaling modulator, was found to increase hair follicle numbers and hair growth in animal models, suggesting its application for AGA. SM04755, a Wnt pathway inhibitor, demonstrated anti-inflammatory and anti-proliferative effects in vitro and improved skin health in a PSO mouse model. Fisetin, a dietary flavonoid, showed in vitro and in vivo potential for treating psoriasis by promoting differentiation, inhibiting proliferation, and modulating inflammatory responses.