The Investigation of LPA4 Functions in Zebrafish

The study investigated the role of the LPA4 receptor in zebrafish, which was structurally distinct from LPA1–3 receptors. Researchers used microinjection of LPA4 morpholino on one-cell stage zebrafish embryos and observed several phenotypes. These included edema around the pericardial region, suggesting vascular leakage or lymphatic vessel failure, a significant decrease in heartbeats with slowed blood flow, and a slight decrease in intersegmental vessel numbers, indicating abnormal blood vessel development. The study concluded that LPA4 might regulate blood and lymph vessel development in zebrafish.