Constitutive Internalization of the Leucine-Rich G Protein-Coupled Receptor-5 (LGR5) to the Trans-Golgi Network
February 2013
in “
Journal of Biological Chemistry
”
TLDR LGR5 mainly stays inside cells, moving to the trans-Golgi network, and this process is important for its role in cell signaling.
The study investigated the internalization and trafficking of the Leucine-rich G Protein-coupled Receptor-5 (LGR5), a stem cell marker in tissues like hair follicles, to the trans-Golgi network (TGN). It was found that LGR5 is constitutively internalized in an agonist-independent manner, primarily through a unique motif involving serine residues 861 and 864, rather than the typical TSSS motif. This process was independent of β-arrestin and involved retrograde trafficking through specific endosomes. The study highlighted the potential novel roles of LGR5 in Wnt pathway signaling and its impact on receptor signaling, stem cell fate, or tumorigenesis, suggesting further research to understand the kinases involved in LGR5 phosphorylation.
