Leucine-Activated Nanohybrid Biofilm for Skin Regeneration via Improving Cell Affinity and Neovascularization Capacity

The study developed a biodegradable nanofibrous biofilm incorporating leucine and LAPONITE® nanodisks to enhance skin regeneration by improving cell affinity and neovascularization. The biofilm was created by doping leucine, which aids fibroblast proliferation, into LAPONITE® nanodisks, which are rich in Mg and Si, promoting vascularization. These nanoparticles were combined with a polylactide (PLA) mesh, treated to improve hydrophilicity, thus enhancing cell affinity. The resulting biofilm supported fibroblast adhesion, migration, and proliferation, facilitating wound healing with blood vessel and hair follicle regeneration, attributed to its improved hydrophilicity and sustained leucine release.