Concentrated Conditioned Medium-Loaded Silk Nanofiber Hydrogels with Sustained Release of Bioactive Factors to Improve Skin Regeneration
September 2019
in “
ACS Applied Bio Materials
”
silk nanofiber hydrogel conditioned medium bioactive proteins TGF-ß1 IGFBP-1 PDGF-AB cell proliferation cell migration fibroblast differentiation myofibroblasts wound healing neovascularization hair follicle regeneration transforming growth factor beta 1 insulin-like growth factor-binding protein 1 platelet-derived growth factor AB
TLDR The hydrogel with bioactive factors improves skin healing and regeneration.
The document describes a study on the development of an injectable silk nanofiber hydrogel loaded with concentrated conditioned medium (CCM) to enhance skin regeneration. The hydrogel was engineered to release bioactive proteins such as TGF-ß1, IGFBP-1, and PDGF-AB over a period of more than 9 days, which promoted cell proliferation and migration, and potentially reduced scar formation by inhibiting fibroblast differentiation into myofibroblasts. In vivo experiments using mice showed that the hydrogel improved wound healing, neovascularization, and hair follicle regeneration. The study concluded that the CCM-loaded hydrogels have a positive impact on skin regeneration and could be a promising approach for clinical wound repair. The document does not mention the number of human subjects as the study was based on in vitro and animal models.
