Keratinocyte Culture Techniques in Medical and Scientific Applications

The document discussed the advancements in keratinocyte culture techniques for treating burn patients, highlighting the use of cultured epithelial autografts (CEA) as an alternative for those with limited donor skin. Since the 1980s, autologous keratinocytes have been used to improve outcomes in burn treatment, although challenges such as high costs, infrastructure needs, and technical issues like infection susceptibility and delayed skin maturation persisted. Despite these challenges, CEA allowed significant expansion of epidermal cells, enabling coverage of large skin defects. Innovations included grafting keratinocyte suspensions and aerosol spraying techniques, although issues with stability and scarring in treated wounds remained.