Protective Effect of Iris Germanica L. Rhizome-Derived Exosome Against Oxidative-Stress-Induced Cellular Senescence in Human Epidermal Keratinocytes
October 2023
in “
Applied sciences
”
Iris germanica L. rhizome-derived exosomes oxidative stress cellular senescence human epidermal keratinocytes reactive oxygen species antioxidant enzyme senescence-associated β-galactosidase cell cycle arrest marker p21 wound-healing properties keratinization markers keratin 1 keratin 10 Iris-exosomes ROS SA-β-gal p21
TLDR Iris germanica rhizome-derived exosomes help protect skin cells from oxidative stress and aging.
The study demonstrates that exosomes derived from Iris germanica L. rhizomes exhibit significant antioxidant and anti-senescence properties in human epidermal keratinocytes (nHEKs). These exosomes, characterized by Cryo-TEM and NTA, improve cell viability, reduce oxidative stress, and decrease cellular senescence markers when nHEKs are exposed to H2O2. They upregulate antioxidant enzymes (HO-1, CAT, GSS, GPx1, and SOD) and modulate the p38/JNK MAPK pathway to lower p53 and p21 levels. Additionally, Iris-exosomes enhance keratinocyte barrier function and wound healing, suggesting potential therapeutic applications for skin aging and oxidative stress-related conditions.
