Industrial-Scale Processing of Activated Platelet-Rich Plasma from Specific Pathogen-Free Pigs and Its Effect on Promoting Human Hair Follicle Dermal Papilla Cell Cultivation

The study aimed to optimize the production of porcine platelet-rich plasma (PRP) for industrial-scale use as a replacement for fetal bovine serum (FBS) and to evaluate its effects on human hair follicle dermal papilla cells (HFDPCs). The researchers developed a process to produce large quantities of porcine PRP, which was activated using collagen, thrombin, and calcium chloride. The results indicated that porcine PRP had higher growth factor levels than FBS and remained stable under various treatments. However, HFDPCs treated with porcine PRP showed no significant difference in proliferation compared to those treated with FBS. The study concluded that porcine PRP could be a commercially viable alternative to FBS and a potential promoter of hair growth.