Design of an In Vitro Skin 3D Model Mimicking a Disruption of the Barrier Function
September 2017
in “
Journal of Investigative Dermatology
”
New to Hyaluronic Acid? There is a guide in the encyclopedia. Read the guide → Studysummary This study suggests that activating the hexosamine pathway may enhance skin homeostasis, potentially increasing hair follicle stem cells and modifying extracellular matrix components like hyaluronic acid in vitro. Our plain-language summary of this paper — not a Tressless recommendation.
This study developed a 3D in vitro model of Reconstructed Epidermis (RE) to mimic disrupted barrier function using normal human keratinocytes. The model was treated with 0.02% sodium lauryl sulfate (SLS) to induce stress, and transcriptomic analysis revealed 929 genes significantly modulated in stressed RE compared to control, with 473 down-regulated and 456 up-regulated. These changes mirrored those in subjects with disrupted barrier function, highlighting 6 over-represented pathways: inflammation, epidermis development, adhesion system, lipids metabolism, defense system, and transport system. The expression of 9 key biomarkers was validated, suggesting the model's potential as a tool for screening therapeutic molecules to repair barrier function.
