In Vitro Cultivation of Dermal-Epidermal Composites in a Single Medium Supports Human Hair Follicle Neogenesis in Xenografts

August 2024 in “ Journal of Investigative Dermatology ”

D.C. Aduba, Habib Md Reazaul Karim, William P. Meehan, Sandhya Xavier, Ao Wang, Jochen Betz, Victor W. Ho, Thomas N. Darling

dermal-epidermal composites human hair follicle neogenesis xenografts PRIME AIRLIFT epidermalization cornification human dermal papilla cells neonatal foreskin keratinocytes bioprinting 3D constructs hair regeneration PA medium DECs HDP cells HK cells

TLDR A single medium, PRIME AIRLIFT, supports better human hair follicle formation in grafts.

The study evaluated the effectiveness of a single commercially available medium, PRIME AIRLIFT (PA), versus sequential use of epidermalization (epi) and cornification (cori) media for cultivating dermal-epidermal composites (DECs) capable of human hair follicle neogenesis in a xenograft mouse model. Human dermal papilla cells (HDP) and neonatal foreskin keratinocytes (HK) showed higher viability and activity in PA medium compared to epi or cori media. Ten DECs were grafted into mice using each method, with 7/10 grafts surviving up to nine weeks in both groups. Human hair follicle formation was observed in 4/7 DECs using epi-cori and 6/7 using PA. The results suggest that PA medium supports better hair follicle formation, indicating potential for improved bioprinting of 3D constructs for hair regeneration.

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