Hypoxic Preconditioned Nanofat at 1% O2 for 24 Hours Loses Its Regenerative In Vivo Vascularization Capacity

This study investigated the effects of hypoxic preconditioning on nanofat from donor mice, exposing it to 1% O₂ for 24 hours. While this preconditioning upregulated certain gene expressions and showed a pro-angiogenic protein profile, it unexpectedly resulted in reduced functional microvessel density in vivo compared to non-preconditioned nanofat. These findings indicate that this specific hypoxic preconditioning protocol does not enhance the regenerative vascularization capacity of nanofat. The study suggests exploring milder preconditioning protocols with shorter hypoxia periods or higher oxygen levels in future research.