High-performance liquid chromatographic method for the determination of finasteride in human plasma at therapeutic doses

May 1991 in “Journal of Chromatography B: Biomedical Sciences and Applications”

M.L Constanzer, Bogdan J. Matuszewski, W.F. Bayne

TLDR Reliable method detects finasteride in human plasma at low doses.

This document describes a validated high-performance liquid chromatographic method for detecting finasteride in human plasma at therapeutic doses. The method was found to be reliable and efficient for quantifying finasteride in human plasma at doses down to 5 mg. The authors also discuss the challenges of detecting finasteride using HPLC due to its low UV absorbance and lack of a functional group for derivatization. Overall, the method was found to be practical and highly reliable.

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