Hair Strengthening Evaluation of Anisotropic Osmolyte Solutions (Inositol + Arginine): Cross-Talk Between Dermal Papilla Fibroblast and Keratinocytes of the Outer Root Sheath Using a Micro Hair Follicle 3D Model

The study aimed to evaluate the biological activity of anisotropic osmolyte solutions (Inositol + Arginine) on the hair follicle (HF) cycle, focusing on the interaction between dermal papilla fibroblast (DPF) and keratinocytes of the outer root sheath (ORSK). The researchers utilized a 3D micro-hair follicle (µHF) model created using hanging drop technology for a DPF-ORSK co-culture, which allowed for a more accurate representation of cell distribution and interaction. This model demonstrated the ability to assess the effectiveness of the osmolyte solutions in improving follicle turnover and health. Additionally, an in silico model was employed to identify the most promising osmolyte combinations, and the findings were validated through in vivo evaluations on volunteers with hair disorders.