Evaluation of the Phenotypic, Proliferative, and Secretory Profile of Mesenchymal Stem Cells from Adipose Tissue Harvested by Laser-Assisted Liposuction

This study evaluated mesenchymal stem cells from adipose tissue (CTM-TA) obtained via laser-assisted liposuction with selective photo-stimulation, comparing them to those obtained through conventional liposuction. Eight volunteers participated, with four undergoing each method. Both groups showed high expression of mesenchymal markers and low expression of hematopoietic markers, confirming their identity as CTM-TA. The cells demonstrated multipotent differentiation into adipocytes, osteoblasts, and chondroblasts. Gene expression analysis revealed a significant increase in ALPL expression in the conventional group. The laser-assisted group showed reduced activity in the G0/G1 phase and increased activity in the S phase of the cell cycle, with no difference in cell cycle progression proteins or cytokine expression between groups. The findings suggest that CTM-TA from laser-assisted liposuction are comparable in differentiation potential and safety for cell therapy applications to those from conventional methods.