TLDR Choosing the right method to separate skin layers is key for good skin cell research.
The document reviews various methods for separating the epidermis and dermis in human skin samples, crucial for skin-related research. The methods are enzymatic, using proteolytic enzymes like dispase for high yields of viable cells; chemical, using neutral salts or acids, suitable for preparing high-quality RNA and DNA or for imaging; and mechanical, each with unique benefits and limitations. It also discusses in vivo and ex vivo skin suction, heat treatment, milling cutter treatment, and stretching. The document provides protocols for skin sample excision, processing, and analysis, and imaging of epidermal and dermal sheets. The choice of separation method depends on the specific experimental question being addressed.
11 citations
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February 2022 in “Scientific Reports” 
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September 2018 in “Scientific reports” Scientists created keratinocyte cell lines from human hair that can differentiate similarly to normal skin cells, offering a new way to study skin biology and diseases.
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February 2018 in “Experimental Dermatology” Scientists developed a way to isolate sweat glands from the scalp during hair transplants, keeping them alive for 6 days for research and cosmetic uses.
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January 2018 in “Journal of Investigative Dermatology” Researchers found four distinct fibroblast types in human skin, which could help in treating wounds and fibrotic diseases.
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November 2014 in “Molecular immunology” Porcine skin is very similar to human skin, making it a useful model for research.
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December 2013 in “International Journal of Dermatology” The document concludes that hair is complex, with a detailed growth cycle, structure, and clinical importance, affecting various scientific and medical fields.
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September 1993 in “British journal of dermatology/British journal of dermatology, Supplement” A new method helps isolate key hair components to study hair growth and loss.
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August 1992 in “PubMed” The rat vibrissa follicle can quickly remodel its basement membrane during hair growth, affecting cell signaling and activity.
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