TLDR Choosing the right method to separate skin layers is key for good skin cell research.
The document reviews various methods for separating the epidermis and dermis in human skin samples, crucial for skin-related research. The methods are enzymatic, using proteolytic enzymes like dispase for high yields of viable cells; chemical, using neutral salts or acids, suitable for preparing high-quality RNA and DNA or for imaging; and mechanical, each with unique benefits and limitations. It also discusses in vivo and ex vivo skin suction, heat treatment, milling cutter treatment, and stretching. The document provides protocols for skin sample excision, processing, and analysis, and imaging of epidermal and dermal sheets. The choice of separation method depends on the specific experimental question being addressed.
16 citations,
September 2018 in “Scientific reports” Scientists created keratinocyte cell lines from human hair that can differentiate similarly to normal skin cells, offering a new way to study skin biology and diseases.
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July 2015 in “Molecular immunology” Porcine skin is very similar to human skin, making it a useful model for research.
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December 2013 in “International Journal of Dermatology” The document concludes that hair is complex, with a detailed growth cycle, structure, and clinical importance, affecting various scientific and medical fields.
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September 1993 in “British journal of dermatology/British journal of dermatology, Supplement” A new method helps isolate key hair components to study hair growth and loss.
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January 2016 in “Elsevier eBooks” The skin is the largest organ, protecting the body, regulating temperature, and producing hormones.
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May 2021 in “International Journal of Molecular Sciences” Cheonggukjang may help prevent and manage various diseases and improve overall health, but its odor and safety concerns need addressing.
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January 2012 in “Elsevier eBooks” The document concludes that various skin conditions have specific treatments, ranging from antihistamines for urticaria to surgery and medication for tumors and chronic skin diseases.
July 2023 in “International Journal of Cosmetic Science” Biopolymers are increasingly used in cosmetics for their non-toxicity and skin benefits, with future biotech advancements likely to expand their applications.
3 citations,
July 2008 in “British journal of dermatology/British journal of dermatology, Supplement” Terbinafine can cause hair loss.
