Detection of Hair MicroRNAs as Novel Potent Biomarkers: Evaluation of Usefulness for Scleroderma Diagnosis

This study evaluated the potential of hair microRNAs as biomarkers for diagnosing scleroderma. Researchers collected hair roots and shafts from 11 scleroderma patients and 13 normal subjects, extracting microRNAs using various methods. They found that microRNAs in hair roots and shafts were detectable and quantifiable, with consistent levels across different head locations. PCR analysis showed distinct expression patterns between serum, hair roots, and hair shafts, suggesting they could serve as independent biomarkers. Notably, miR-196a levels in hair shafts were significantly lower in scleroderma patients compared to normal subjects (p<0.05), indicating that hair microRNAs might be effective and accessible biomarkers for scleroderma diagnosis.