Cyclic ADP-Ribose Modulates Intracellular Calcium Homeostasis and Anagen-Associated Signaling Pathways in Human Hair Follicle Dermal Papilla Cells

The study examined the effects of cyclic ADP-ribose (cADPR) on human hair follicle dermal papilla cells, focusing on intracellular calcium retention and anagen-associated signaling pathways. cADPR treatment, particularly at 0.05 ppm, increased intracellular calcium levels and enhanced β-catenin-dependent transcriptional activity, with a 2.3-fold increase in β-catenin transcription and a 2.5-fold upregulation of LEF-1 expression. It also resulted in a 0.3-fold downregulation of TGF-β2 expression. These findings suggest that cADPR modulates intracellular calcium dynamics and anagen-related signaling, indicating its potential influence on hair follicle biology. However, the study does not establish a direct causal relationship between calcium mobilization and β-catenin activation, highlighting the need for further research into its mechanistic and therapeutic potential.