Efficient Editing of CSLD2 Orthologue by CRISPR/Cas9 Affects Cell Morphogenesis of Root Hair in Spinach
January 2022
in “
Research Square (Research Square)
”
CRISPR/Cas9 cellulose synthase-like D CSLD Spo23361 Spo10340 root hair formation transgenic hairy root lines homozygous bi-allelic heterozygous chimeric Arabidopsis csld2 mutants cell wall modulation membrane trafficking Agrobacterium rhizogenes CRISPR root hair transgenic roots Arabidopsis mutants cell wall membrane
Preprint — not peer reviewed. This was posted to a preprint server or data repository. It has not been through a journal's review process, and its findings may change or not hold up.
Studysummary This study found that CRISPR/Cas9 efficiently edited two cellulose synthase-like genes in spinach, significantly altering root hair growth patterns and suggesting potential for large-scale genome editing in this crop.
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