Chondrogenic Differentiation of Human Scalp Adipose-Derived Stem Cells in Polycaprolactone Scaffold Using Freeze Thaw Freeze Method

January 2018 in “ Journal of Cellular Physiology ”

Rana Moradian Tehrani, Hamed Mirzaei, Javad Verdi, Amirhossein Sahebkar, Mahdi Noureddini, Roya Salehi, Behrang Alani, Mojtaba Kianmehr

human scalp adipose-derived stem cells Polycaprolactone Freeze Thaw Freeze method SADSCs TGF-beta3 BMP-6 collagen type II aggrecan SOX9 flow cytometry histology immunohistology real-time PCR PCL FTF stem cells growth factors TGF-beta BMP

TLDR Human scalp fat stem cells showed improved cartilage-like development on a special scaffold with freeze-thaw treatment.

The 2018 study investigated the potential of human scalp adipose-derived stem cells (SADSCs) for chondrogenic differentiation using a Polycaprolactone (PCL) scaffold combined with a Freeze Thaw Freeze (FTF) method. The SADSCs were characterized and found to express specific surface markers (CD90, CD44, and CD105) and not CD45. When induced with growth factors TGF-beta3 and BMP-6, the cells showed increased expression of chondrogenic markers (collagen type II, aggrecan, and SOX9) and enhanced differentiation and proliferation on PCL scaffolds with FTF treatment compared to PCL alone. These findings suggest that the PCL+FTF scaffold could be a promising approach for cartilage tissue engineering. The study included various methods such as flow cytometry, histology, immunohistology, and real-time PCR, and the results were statistically significant. However, the number of people from whom the SADSCs were isolated was not mentioned.

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