TLDR New techniques helped identify rare wool proteins by reducing dominant ones.
The study focused on characterizing low-abundance wool proteins, specifically keratin associated proteins (KAPs), which were difficult to identify due to the dominance of intermediate filament proteins (IFPs) in wool fibres. The research addressed the limited sequence information available for wool KAPs compared to human hair by acquiring new sequences. By employing novel differential extraction techniques based on solubility with pH, urea, and DTT concentration, the researchers significantly reduced the concentration of IFPs. This advancement allowed for the identification of low-abundance proteins in 2-D electrophoretic maps, enhancing the understanding of the wool proteome.
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