Transport of Biotin in Human Keratinocytes

March 2003 in “ Journal of Investigative Dermatology ”

Franziska Grafe, W Wohlrab, Reinhard H.H. Neubert, Matthias Brandsch

biotin keratinocytes sodium-dependent biotin transport HaCaT cells lipoic acid pantothenic acid desthiobiotin biocytin biotin methyl ester Na+-dependent multivitamin transporter pantothenate vitamin B7 vitamin H skin cells sodium-dependent vitamin transport alpha-lipoic acid vitamin B5 biotin precursor biotin ester

TLDR Human skin cells have a high-affinity system for biotin transport, crucial for skin health.

The study examined biotin transport in human keratinocytes, revealing a sodium-dependent, high-affinity biotin transport system in HaCaT cells with a Michaelis-Menten constant of 22.7±1.0 μM and a maximal velocity of 163.6±3.5 pmol per 5 min per mg protein. Biotin uptake was enhanced by an Na+ gradient and inhibited by lipoic acid, pantothenic acid, and desthiobiotin, but not by biocytin or biotin methyl ester. The findings indicated that keratinocytes express a Na+-dependent multivitamin transporter with a preference for pantothenate and a high-affinity component for biotin, highlighting biotin's importance in skin health and potential therapeutic applications.

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Transport of Biotin in Human Keratinocytes

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