Transport of Biotin in Human Keratinocytes
March 2003
in “
Journal of Investigative Dermatology
”
biotin keratinocytes sodium-dependent biotin transport HaCaT cells lipoic acid pantothenic acid desthiobiotin biocytin biotin methyl ester Na+-dependent multivitamin transporter pantothenate vitamin B7 vitamin H skin cells sodium-dependent vitamin transport alpha-lipoic acid vitamin B5 biotin precursor biotin ester
TLDR Human skin cells have a high-affinity system for biotin transport, crucial for skin health.
The study examined biotin transport in human keratinocytes, revealing a sodium-dependent, high-affinity biotin transport system in HaCaT cells with a Michaelis-Menten constant of 22.7±1.0 μM and a maximal velocity of 163.6±3.5 pmol per 5 min per mg protein. Biotin uptake was enhanced by an Na+ gradient and inhibited by lipoic acid, pantothenic acid, and desthiobiotin, but not by biocytin or biotin methyl ester. The findings indicated that keratinocytes express a Na+-dependent multivitamin transporter with a preference for pantothenate and a high-affinity component for biotin, highlighting biotin's importance in skin health and potential therapeutic applications.
