Biochemical Analysis of Hair Growth from the Aspects of Aging and Enzyme Activities

The study investigated hair regrowth in C3H mice of varying ages by analyzing enzyme activities in different skin areas after shaving. It was found that hair regrowth was irregular and delayed as mice aged, with older mice showing more significant delays. In 59-day-old mice, 30-50% of the shaved area was covered with new hair nine days post-shaving. Biochemical analysis revealed that enzyme activities of ornithine decarboxylase (ODC), transglutaminase (TGase), and alkaline phosphatase (Al-P) were significantly higher in hair re-growing areas compared to non-growing areas. These enzyme activities were also elevated in follicle-rich fractions from re-growing areas compared to non-shaved areas, suggesting a link between these enzymes and hair growth.