Analysis of changes in microRNA expression profiles in response to the troxerutin-mediated antioxidant effect in human dermal papilla cells

The study explored the effects of troxerutin, a flavonoid derivative, on human dermal papilla cells under oxidative stress from hydrogen peroxide (H₂O₂). Troxerutin pretreatment significantly reduced H₂O₂-induced cytotoxicity, cell death, and reactive oxygen species (ROS) production. It also decreased the number of cells in the sub-G1 phase and inhibited senescence-associated β-galactosidase activity. MicroRNA (miRNA) analysis revealed 24 differentially expressed miRNAs, which are implicated in various biological functions and signaling pathways related to aging, skin development, apoptosis, and cell proliferation. The study concluded that troxerutin has potential as a protective agent against oxidative stress in HDP cells and may be beneficial for treating hair loss conditions like alopecia. The experiments were conducted in triplicate, and statistical significance was indicated by P<0.05. However, the specific number of cells or participants used in the study was not mentioned in the summary.