Characterization and Localization of Side Population Cells in Mouse Skin

May 2005 in “ Stem Cells ”

Shoichiro Yano, Yuriko Ito, Manabu Fujimoto, Tatsuo S. Hamazaki, Kunihiko Tamaki, Hitoshi Okochi

side population cells epidermis alpha6-integrin beta1-integrin Sca-1 keratin 14 keratin 19 E-cadherin CD34 CD71 breast cancer resistance protein 1 BCRP1 keratinocyte stem cells hair bulge SP cells skin stem cells

Studysummary This study found that keratinocyte stem cells in mouse skin are closely related to side population or BCRP1-positive cells based on their localization and marker expression.

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The study identified side population (SP) cells in mouse skin, which were primarily located in the epidermis and decreased with age. These cells, characterized by their ability to efflux Hoechst 33342 dye, expressed high levels of alpha6-integrin, beta1-integrin, Sca-1, keratin 14, and keratin 19, and low levels of E-cadherin, CD34, and CD71. The breast cancer resistance protein 1 (BCRP1), crucial for dye efflux, was highly expressed in SP cells and localized in the basal layers and hair bulge regions, aligning with keratinocyte stem cells. The findings suggested a close relationship between keratinocyte stem cells and SP- or BCRP1-positive cells.

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