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Glossary Ultra-Performance Liquid Chromatography Tandem Mass Spectrometry

advanced technique for separating and identifying compounds in samples

Ultra-Performance Liquid Chromatography Tandem Mass Spectrometry (UPLC-MS/MS) is an advanced analytical technique used to separate, identify, and quantify compounds in a sample. UPLC enhances the resolution and speed of liquid chromatography, while MS/MS provides highly sensitive and specific detection by breaking down molecules into fragments and analyzing their mass. This method is widely used in fields like pharmacology, biochemistry, and environmental science for precise and accurate analysis of complex mixtures.

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Simultaneous Determination of Androgens and Prostaglandins in Human Urine Using Ultra-High-Performance Liquid Chromatography–Tandem Mass Spectrometry

research Simultaneous determination of androgens and prostaglandins in human urine using ultra-high-performance liquid chromatography–tandem mass spectrometry

10 citations , January 2019 in “Journal of Chromatography B”

This study used a newly validated method to find that one-year finasteride treatment resulted in no significant differences in urinary androgen and prostaglandin levels between male pattern baldness patients and normal controls.

research Determination of 13 janus kinase inhibitors in anti-alopecia cosmetics by ultra-high performance liquid chromatography-tandem triple quadrupole composite linear ion trap mass spectrometry

September 2025 in “Chinese Journal of Chromatography”

This study developed a method using ultra-high performance liquid chromatography-mass spectrometry (UHPLC-MRM-IDA-EPI) for the rapid and sensitive detection of 13 JAK inhibitors in anti-alopecia cosmetics, showing excellent separation and accurate quantification with high recovery rates in tested matrices.

research Simultaneous determination of androgens and prostaglandins in human urine using ultra-high-performance liquid chromatography–tandem mass spectrometry

January 2019

This study found no significant difference in urinary androgen and prostaglandin levels between male pattern baldness patients treated with finasteride for one year and normal controls.

Phytochemical Analyses of Ziziphus Jujuba Mill. Var. Spinosa Seed by Ultrahigh Performance Liquid Chromatography-Tandem Mass Spectrometry and Gas Chromatography-Mass Spectrometry

research Phytochemical analyses of Ziziphus jujuba Mill. var. spinosa seed by ultrahigh performance liquid chromatography-tandem mass spectrometry and gas chromatography-mass spectrometry

38 citations , January 2013 in “Analyst”

This study identified the main secondary metabolites in Ziziphus jujuba seeds and confirmed that UPLC-MS/MS and GC-MS effectively demonstrate their pharmaceutical quality.

research A quantitative ultra-performance liquid chromatography high-resolution mass spectrometry analysis of steroids from human scalp hair

7 citations , April 2022 in “Journal of pharmaceutical and biomedical analysis”

This study describes a new method using high-resolution mass spectrometry for analyzing multiple steroids in human scalp hair, with newborn hair showing the highest steroid concentrations among examined age groups.

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Craig Bottomley-High
Studham, England, United Kingdom
13+ yrs

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Craig Bottomley-High