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GlossaryImmunostaining (Immunofluorescence)

technique using antibodies to detect specific proteins in tissues

Immunofluorescence (IF) is a laboratory technique used to visualize the presence and location of specific proteins or antigens in cells or tissue sections by using antibodies that are chemically linked to a fluorescent dye. When these antibodies bind to their target, the fluorescent dye can be seen under a microscope with the appropriate light, allowing researchers to study the distribution and abundance of the proteins of interest.

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research Immunofluorescence Analysis of the Basement Membrane Zone Components in Human Anagen Hair Follicles

29 citations , July 2003 in “Experimental Dermatology”

The upper hair follicle is stable, while the lower part allows movement during hair growth.

research Immunofluorescence Tomography: High-Resolution 3D Reconstruction by Serial-Sectioning of Methacrylate Embedded Tissues and Alignment of 2D Immunofluorescence Images

8 citations , February 2019 in “Scientific Reports”

Immunofluorescence tomography is a cost-effective method for creating detailed 3-D images of tissues.

research Detection of Bim and Puma in Mouse Hair Follicles Using Immunofluorescence and TUNEL Assay Double Staining

3 citations , July 2015 in “Biotechnic & histochemistry”

Bim and Puma proteins are found in developing mouse hair follicles and are involved in more than just cell death.

Co-Expression Pattern of Artemis Serine 516 Phosphorylation with Indicators in Differentiation, Proliferation, and Apoptosis of Human Anagen Hair Follicles: An Immunofluorescence Double-Staining Study

research Co-Expression Pattern of Artemis Serine 516 Phosphorylation with Indicators in Differentiation, Proliferation, and Apoptosis of Human Anagen Hair Follicles: An Immunofluorescence Double-Staining Study

January 2023 in “Frontiers in bioscience”

Artemis protein may help control hair growth and health by influencing cell processes.

research Clinical Significance of Dense Fine Speckled Pattern in Anti-nuclear Antibody Test Using Indirect Immunofluorescence Method

28 citations , April 2009 in “Annals of laboratory medicine”

The dense fine speckled pattern in ANA tests is common in autoimmune diseases, challenging previous beliefs that it was unrelated.

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