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This study found that using trypsin digestion to extract keratinocytes from the hair follicle bulge results in a higher yield and faster growth of stem keratinocytes compared to the explant method.

This review suggests that omitting the proteolysis step and including tissue pieces in mesenchymal stem cell isolation may improve cell quality, reduce contamination risk, and lower costs and processing time in clinical applications.

This study found that an enzymatic digestion method for isolating hair follicle stem cells from scalp specimens yielded 5.3 times more epithelial cells than explant cultures, while supporting comparable cell functionality, offering a promising technique for tissue engineering and regenerative medicine.

This study presents an improved protocol for isolating and culturing whole neonatal cochlear explants, enabling detailed analysis of hair cells and spiral ganglion neuron cells while simplifying culture steps without sacrificing explant quality, and was successfully tested on mice and rat cochleas.

This study characterized human Wharton's Jelly Mesenchymal Stem Cells (hWJ-MSCs) isolated from umbilical cords using explant and enzymatic methods, finding both methods yielded high-purity cells capable of differentiating into adipocyte, chondrocyte, and osteocyte lineages, showing promise for regenerative medicine applications.