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In this study, the authors reported that taking 20 mg or more of biotin can interfere with thyroid function tests, potentially leading to false diagnoses of conditions like Grave's disease.

This study presents a method using liquid chromatography tandem mass spectrometry to quantify biotin in plasma and serum, and explores how high biotin intake can interfere with diagnostic immunoassays that utilize biotin-streptavidin technology, potentially leading to misdiagnosis of endocrine disorders.

This case report describes a girl with Denys-Drash syndrome misdiagnosed with hyperandrogenism due to biotin interference in immunoassays, highlighting the need for awareness of laboratory result discrepancies.

This review discusses biotin supplementation for dermatological conditions and highlights the lack of strong evidence supporting its efficacy, as well as the potential for laboratory testing interference.

This study identified that biotin ingestion led to interference in immunoassay results, falsely indicating high testosterone and insulin levels in a 28-year-old female patient, thus complicating her clinical assessment and treatment planning until recognized and resolved.