Biocompatible ZnO Nanoflower-Infused Chitosan/Alginate/PVA Composite for Accelerated Skin Regeneration

This study presents a biocompatible zinc oxide nanoflower (ZnO NF)-infused chitosan/alginate/polyvinyl alcohol (Cs/Alg/PVA) nanocomposite designed to accelerate skin regeneration. The ZnO NFs were synthesized using a green method and incorporated into the composite, which showed excellent swelling capacity, controlled release, and mechanical stability. In vitro tests with HDF and L929 cell lines confirmed non-cytotoxicity and enhanced cell proliferation, while hemocompatibility assessments indicated minimal hemolytic activity. In vivo studies in a murine model demonstrated faster wound healing, improved angiogenesis, reduced inflammation, and better collagen deposition in ZnO NF-treated groups. These results suggest that ZnO NF-based biopolymers hold significant potential for use in advanced wound dressings and tissue engineering.