A Volar Skin Excisional Wound Model for In Situ Evaluation of Multiple-Appendage Regeneration and Innervation

The study developed a volar skin excisional wound model (VEWM) using 40 C57BL/6 mice to examine cutaneous wound healing, multiple-appendage restoration, and innervation. The VEWM closely mimics the healing process of human wounds, with wound areas at 1, 3, 7, and 10 days after operation being 89.17%, 71.72%, 55.09%, and 35.74% respectively, and the final scar area accounting for 47.80% of the initial wound. The volar skin of the mice, richly innervated and comprising hair follicles, sebaceous glands, and sweat glands, was studied. Hair growth was limited to the inter-footpad area, with 67.23 hairs on average. The number of sweat glands in footpads and inter-footpads was 77.25 and 3.95 respectively. The study concluded that the VEWM can be used for skin multiple-appendages regeneration and innervation evaluation.