Type 2 Cytokine-Dependent Skin Barrier Regulation in Personalized 2D and 3D Skin Models of Atopic Dermatitis: A Pilot Study

August 2024 in “ JID Innovations ”

Hila Emmert, Franziska Rademacher, Matthias Hübenthal, Regine Gläser, Hanne Norsgaard, Stephan Weidinger, Jürgen Harder

keratinocytes hair follicles atopic dermatitis type 2 cytokines IL-4 IL-13 epidermal spongiosis skin barrier AD inflammation skin models

TLDR AD-derived keratinocytes effectively mimic inflammation in atopic dermatitis.

This pilot study explored the effects of type 2 cytokines IL-4 and IL-13 on atopic dermatitis (AD) using personalized 2D and 3D skin models derived from keratinocytes (KCs) of AD patients. The study found that these cytokines, especially IL-13, significantly altered the expression of AD-associated genes and proteins, impacting skin barrier function. IL-13 alone induced spongiosis-like structures, a hallmark of AD, suggesting its dominant role in AD pathogenesis. The research supports using these in vitro models to study AD mechanisms and optimize treatments, highlighting IL-13 as a potential therapeutic target.

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