Production of a Skin Equivalent Using Human Outer Root Sheath Cells and Fibroblasts
October 2007
TLDR A skin model using hair and skin cells can mimic human skin for research.
The study demonstrated a method for creating a skin equivalent (SE) using human outer root sheath cells (ORSCs) and fibroblasts. ORSCs were isolated from hair follicles and cultured, then combined with fibroblasts in a collagen-GAG matrix to form a dermal equivalent. This SE was transplanted into mice, where it developed into a multi-layered, cornified epidermis within 2 weeks, resembling human skin. The findings indicated that the isolation and culturing technique for ORSCs was effective, and the SE could form structures similar to human dermis and epidermis, providing a potential model for skin regeneration research.
