TLDR A method was developed to grow millions of hair cells from a single hair for research and storage.
The document described a method for the serial cultivation of adult human hair follicle keratinocytes using plucked scalp hair follicles placed on bovine eye lens capsules. This method allowed for the rapid expansion of keratinocyte colonies, which could be subcultured up to four times, generating approximately 10 million keratinocytes from a single hair follicle. The technique also enabled cryogenic storage for easy transportation and facilitated immunofluorescence studies by plating subcultured keratinocytes on glass coverslips to visualize keratin cytoskeletons.
88 citations
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January 1981 in “British Journal of Dermatology” The document described a pioneering method for culturing human hair follicle cells using the bovine eye lens capsule as a substrate. This approach allowed for the formation of growing colonies of diploid keratinocytes in a culture medium supplemented with hydrocortisone and insulin, with approximately 70% of the original follicles successfully forming these colonies.
50 citations
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July 2008 in “British Journal of Dermatology” 138 citations
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March 2007 in “Experimental cell research” Only a few hair-specific keratins are linked to inherited hair disorders.
276 citations
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January 2005 in “International review of cytology” More research is needed to understand how hair keratins work and their role in hair disorders.
199 citations
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January 2004 in “The International Journal of Developmental Biology” Hair follicle growth and development are controlled by specific genes and molecular signals.
29 citations
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April 2003 in “Experimental dermatology” Human hair follicles grown in vitro maintain normal keratin patterns and structure.
14 citations
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July 1983 in “Acta Dermato Venereologica” A method was developed to grow millions of hair cells from a single hair for research and storage.
