RNA Sequencing of Immortalised Balding and Non-Balding DPCs Identifies Potential Balding Gene Signatures
November 2022
in “
Journal of Investigative Dermatology
”
RNA sequencing balding DPCs non-balding DPCs bi-dimensional culture three-dimensional culture co-culture adipose derived stem cells inflammatory pathways gene profile expression RNA-seq balding dermal papilla cells non-balding dermal papilla cells 2D culture 3D culture co-culture with ASCs fat-derived stem cells inflammation gene expression
Studysummary This study found that non-balding dermal papilla cells emphasized extracellular matrix organization and reduced inflammation when cultured in 3D or with adipose-derived stem cells, unlike balding cells which maintained inflammatory pathways.
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The study compared the gene profile expression in immortalised balding (B-DPCs) and non-balding DPCs (NB-DPCs) in three different culture conditions: bi-dimensional (2D), three-dimensional (3D), and in co-culture with adipose derived stem cells (ASCs). The study also compared primary DPCs in the same three culture conditions in early, mid, and late passages (P3, P6, and P9). RNA sequencing was performed and the analysis showed that B, NB, and primary DPC genes cluster very distinctly. The 3D and co-culture with ASCs conditions create changes in gene profile expression of all DPC types. The balding profile is very distinct from non-balding and primary DPCs and promotes inflammatory pathways in all culture conditions. The study concluded that 3D and co-culture emphasize the maintenance of immortalisation features in NB compared to primary DPCs.
