Development and Evaluation of RADA-PDGF2 Self-Assembling Peptide Hydrogel for Enhanced Skin Wound Healing

November 2023 in “ Frontiers in Pharmacology ”

Milena Deptuła, Justyna Sawicka, Piotr Sass, Piotr Sosnowski, Przemysław Karpowicz, Małgorzata Zawrzykraj, Anna Wardowska, Agata Tymińska, Maria Dzierżyńska, Zuzanna Pietralik-Molińska, Barbara Peplińska, Jacek Zieliński, Karolina Kondej, Maciej Kozak, Paweł Sachadyn, Sylwia Rodziewicz‐Motowidło, Michał Pikuła

RADA-PDGF2 self-assembling peptide hydrogel PDGF2 RADA16-I neutrophil elastase bioactive sequences cell proliferation cell migration wound closure non-cytotoxic non-immunogenic

Studysummary In this study, researchers developed a self-assembling hydrogel called RADA-PDGF2 and found it to accelerate wound closure in a mouse model due to its pro-proliferative and pro-migratory properties, without causing cytotoxicity or immunogenicity.

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The study developed a self-assembling peptide hydrogel, RADA-PDGF2, to enhance skin wound healing by combining the RADA16-I peptide with the PDGF2 peptide, linked by a neutrophil elastase-specific fragment. This hydrogel demonstrated minimal cytotoxicity, promoted cell proliferation and migration, and enhanced collagen synthesis in vitro. In a murine model, it significantly accelerated wound closure and epithelialization compared to a control hydrogel, with 60% closure by day 9 versus 23% in the control. The hydrogel's chemical stability, biocompatibility, and ability to promote wound healing suggest its potential for treating chronic wounds, although further research is needed to optimize its therapeutic applications. The study involved 12 wounds in the mouse model.

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research Release Systems Based on Self-Assembling RADA16-I Hydrogels With a Signal Sequence Which Improves Wound Healing Processes

1 citations , April 2023 in “Scientific Reports”

This study reported that newly developed RADA16-I peptide hybrids, with active motifs like GHK and KGHK, demonstrated improved wound healing in a mouse skin injury model without cytotoxicity, suggesting they might be effective scaffolds for tissue engineering and regeneration.

research Stem Cell Dynamics, Migration, and Plasticity During Wound Healing

211 citations , November 2018 in “Nature Cell Biology”

This article discusses the coordinated roles of different epidermal stem cells and other cell types in the skin's wound healing process and reports no new experimental results.

research Self-Assembling Peptide Hydrogel Scaffolds Support Stem Cell-Based Hair Follicle Regeneration

38 citations , June 2016 in “Nanomedicine: Nanotechnology, Biology and Medicine”

In this study, the RADA-PRG hydrogel enhanced SKP proliferation, survival, and gene expression, effectively supporting hair follicle regeneration in mice.